Selected publications relating to induced heteroduplex generator (IHG) technology

Highlighted publications are downloadable as Adobe PDF files

1 : Bidwell, JL, Clay, TM, Wood, NAP, Pursall, MC, Martin, AF, Bradley, BA and Hui, KM (1993) Rapid HLA-DR-Dw and DP matching by PCR fingerprinting and related DNA heteroduplex technologies. pp 99-116 in: Handbook of HLA Typing Techniques [Eds KM Hui & JL Bidwell] CRC Press: Boca Raton, Florida.

2 : Wood, N, Tyfield, L, Bidwell, J (1993) Rapid classification of phenylketonuria genotypes by analysis of heteroduplexes generated by PCR-amplifiable synthetic DNA. Human Mutation 2: 131-137.

3 : Bidwell, JL, Wood, NAP, Tyfield, LA, Clay, TM, Culpan, D, Evans, JM, Pursall, MC, Bradley, BA (1993) Universal heteroduplex generators: reagents for genotyping of HLA and of human diseases. Molecular Bases of Human Diseases (Ed. E.E.Polli). pp27-34. Elsevier/North-Holland, Amsterdam.

4 : Wood, N, Standen, G, Hows, J, Bradley, B, Bidwell, J. (1993) diagnosis of sickle cell disease with a universal heteroduplex generator. Lancet 342: 1519-1520.

5 : Bidwell, JL, Wood, NAP, Clay, TM, Pursall, MC, Culpan, D, Evans, J, Bradley, BA, Tyfield, LA, Standen, G, Hui, KM (1994) DNA heteroduplex technology. In: Chrambach, A, Dunn, MJ, Radola, BJ (Eds), Advances in Electrophoresis, Volume 7, pp311-351. VCH Press, Weinheim 

6 : Clay, TM, Cuplan, D, Howell, WM, Sage, DA, Bradley, BA, Bidwell, JL. (1994) UHG crossmatching: a comparison with PCR-SSO typing in the selection of HLA-DPB1-compatible bone marrow donors. Transplantation 58: 200-207.

7 : Bidwell, JL. (1994) Advances in DNA-based HLA-typing methods. Immunology Today 15: 303-307.

8 : Tyfield, LA, Stephenson, A, Bidwell, JL, Wood, NAP, Cockburn, F, Harvie, A, Smith, I. (1994) Mutation analysis of the phenylalanine hydroxylase gene using heteroduplex analysis with synthetic DNA constructs. Acta Paediatrica 83: 47-48.

9 : Savage, DA, Wood, NAP, Bidwell, JL, Hui, KM. (1995) HLA-DRB1*01 subtyping by heteroduplex analysis. Tissue Antigens 45: 120-124.

10 : Wood, N, Standen, G, Murray, EW, Lillicrap, D, Holmberg, L, Peake, IR, Bidwell, J. (1995) Rapid genotype analysis in type 2B von Willebrand's disease using a universal heteroduplex generator. Brit J Haematol 89: 152-156 

11 : Wood, N, Standen, G, Old, J, Bidwell, J. (1995) Optimisation and properties of a UHG for genotypng of haemoglobins S and C. Hum Mutation 5: 166-172.

12 : Savage, DA, Wood, NAP, Bidwell, JL, Fitches, A, Old, JM, Hui, KM. (1995) Detection of b-thalassaemia mutations using DNA heteroduplex generator molecules. Brit  J Haematol 90: 564-571.

13 : Tyfield, LA, Zschocke, J, Stephenson, A, Cockburn, F, Harvie, A, Bidwell, JL, Wood, NAP, Hunt, LP. (1995) Discordant phenylketonuria phenotypes in one family: the relationship between genotype and clinical outcome is a function of multiple effects. J Med Genet 32: 132-136.

14 : Wood, NAP, Bidwell, JL. (1996) UHG: heteroduplex and universal heteroduplex generator analysis. In: Laboratory protocols for mutation detection (ed Landegren, U), pp 105-112. Oxford University Press for the Human Genome Organisation.

15 : Wood, N, Bidwell, J. (1996) Genetic screening and testing by induced heteroduplex formation. Electrophoresis 17: 247-254.

16 : Wood, N, Standen, GR, Bowen, DJ, Cumming, A, Lush, C, Lee, R, Bidwell, J. (1996) UHG-based mutation screening in type 2B von Willebrand's disease: detection of a candidate mutation Ser547Phe. Thrombosis & Haemostasis 75: 363-367.

17 : Savage, DA, Tang, JP, Wood, NAP, Evans, J, Bidwell, JL, Wee, JLK, Oei, AA, Hui, KM (1996) A rapid HLA-DRB1*04 subtyping method using PCR and DNA heteroduplex generators. Tissue Antigens 47: 284-292.

18 : Jack, D, Bidwell, J, Turner, M, Wood, N. (1997) Simultaneous genotyping for all three known structural mutations in the human mannan-binding lectin gene. Human Mutation 9: 41-46.

19 : Culpan, D, Standen, GR, Wood, N, Mazurier, C, Gaucher, C, Bidwell, JL (1997) Rapid mutation screening in Type 2A von Willebrand's disease using universal heteroduplex generators. Brit J Haematol 96: 464-469.

20 : Bowen, D, et al (1997) Genetic diagnosis of Factor V Leiden using heteroduplex technology. Thrombosis and Haemostasis 77: 119-122.

21 : Tyfield, LA, Stephenson, A, Cockburn, F, Harvie, A, Bidwell, JL, Wood, NAP, Pilz, DT, Harper, P, Smith, I (1997) Sequence variation at the phenylalanine hydroxylase gene in the British Isles. Am J Hum Genet 60: 388-396

22 : Enayat, MS, Theophilus, BDM, Hill, FGH, Rose, PE, Culpan, D, Bidwell, J, Standen, GR (1998) A new candidate mutation (K755E) causing Type 2A Von Willebrand’s disease identified by UHG analysis. Br J Haematol 79:240

23 : Culpan, D, Goodeve, A, Bowen, DJ, Standen, G, Bidwell, J (1988) Rapid genotypic diagnosis of type 2A von Willebrand’s disease by heteroduplex analysis. Clin. Lab. Haem 20: 177-178.

24 : Bowen, DJ, Standen, GR, Mazurier, C, Gaucher, C, Cumming, A, Keeney, S, Bidwell, J (1998) Type 2N von Willebrand disease: rapid genetic diagnosis of G2811A (R854Q), C2696T (R816W), T2701A (H817Q) and G2823T (C858F) - detection of a novel candidate type 2N mutation: C2801T (R854W). Thromb Haemost  80: 32-36.

25 : Morse, HR, Olomolaiye, OO, Wood, NAP, Keen, LJ, Bidwell, JL (1999) Induced heteroduplex genotyping of TNFa, IL-1b, IL-6 and IL-10 polymorphisms associated with transcriptional regulation. Cytokine 11: 789-795

26 : Bolla, MK, Wood, N, Humphries, SE (1999) Rapid determination of apolipoprotein E genotype using a heteroduplex generator. J Lipid Res 40: 2340-2345

27 : Wood, NAP, Thompson, SC, Smith, RM, Bidwell, JL (2000) Identification of human TGF-b1 signal (leader) sequence polymorphisms by PCR-RFLP. J Immunol Methods 234: 117-122.

28 : Bidwell, JL, Olomolaiye, OO, Keen, LJ, Wood, NAP, Morse, HR, Laundy, GJ, Thompson, SJ (2000) Cytokine gene polymorphism. In: Human Blood Cells: Consequences of Genetic Polymorphism (Ed. King, M-J), pp375-400. Imperial College Press, London.

29 : Wood, NAP, Keen, LJ, Tilley, LA, Bidwell, JL (2001) Determination of cytokine regulatory haplotypes by induced heteroduplex analysis of DNA. J Immunol Meth 249:191-198.

30 : Spink, CF, Keen, LJ, Middleton, PG, Bidwell, JL. Discrimination of suballeles present at the TNFd microsatellite locus using Induced Heteroduplex Analysis. Genes and Immunity 5: 76-79.

31 : Kirkpatrick, A, Bidwell, J , Van den Brule, AJC , Meijer, CJLM, Pawade, J, Glew, S. TNFa polymorphism frequencies in HPV-associated cervical  dysplasia. Gynecological Oncology 92 (2): 675-679

32 : Keen LJ, DeFor TE, Bidwell JL, Davies SM, Bradley BA and Hows JM (2004) Interleukin-10 And Tumor Necrosis Factor Alpha Region Haplotypes Predict Transplant Mortality After Unrelated Donor Stem Cell Transplantation. Blood 103:3599-3602.

33 : Cardoso SP, Keen L, Bidwell J. (2004) Identification of two novel single nucleotide polymorphisms in the promoter of the human IL-18 receptor alpha gene. European Journal of Immunogenetics  31: 27-29

34 : Cardoso SP, Defor TE, Tilley LA, Bidwell JL, Weisdorf DJ, MacMillan ML (2004). Patient Interleukin-18 GCG haplotype associates with improved survival and decreased transplant-related mortality after unrelated-donor bone marrow transplantation. British Journal of Haematology 126(5): 704-710

35 : Wood N, Fraser A, Bidwell J, Standen G (2004) RT-PCR permits simultaneous genotyping of thiopurine S-methyltransferase allelic variants by multiplex induced heteroduplex analysis. Hum Mutat. 24:93-99.

36 : Smith AJP, Keen LJ, Billingham MJ, Perry MJ, Elson CJ, Kirwan JR, Sims JE, Doherty M, Spector TD, Bidwell JL (2004) Extended haplotypes and linkage disequilibrium in the IL1R1–IL1A–IL1B–IL1RN gene cluster: association with knee osteoarthritis. Genes and Immunity 5: 451-460

37 : Morgan GJ, Adamson PJ, Mensah FK, Spink CF, Law GR, Keen LJ, Roman E, Davies FE, Rollinson S, Child JA, Bidwell JL.(2005) Haplotypes in the tumour necrosis factor region and myeloma. Br J Haematol. 2005 May;129(3):358-65.

38 : Medford AR, Keen LJ, Bidwell JL, Millar AB. (2005) Vascular endothelial growth factor gene polymorphism and acute respiratory distress syndrome. Thorax 60:244-248.

39 : Smith AJ, Elson CJ, Perry MJ, Bidwell JL. (2005) Accuracy of haplotype association studies is enhanced by increasing number of polymorphic loci examined: comment on the article by Meulenbelt et al. Arthritis Rheum. 52:675

40 : Atan D, Turner SJ, Kilmartin DJ, Forrester JV, Bidwell J, Dick AD, Churchill AJ. (2005) Cytokine gene polymorphism in sympathetic ophthalmia. Inv Ophthal Visual Sci (in press)

41 : Spink CF, Gray LC, Davies FE, Cohen D, Hawkins K, Morgan GJ, Bidwell, JL (2005) Haplotypic structure across the IκBa gene (NFKBIA) and association with multiple myeloma. Cancer Letters (in press)

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Powerpoint presentation: some applications
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